The ThermoFisher Attune NxT is a clinical style flow cytometer typically used for routine counting and characterization of cells and populations with the major advantage that it has a microplate autosampler and it uses both hydrodynamic and acoustic focusing, which is particularly useful for large dynamic ranges of cell sizes. Its main purpose is for population enumeration and characterization using high throughput sampling. The instruments specifications are as follows:
Lasers
405 nm (“UV”)
488 nm
Optics (user configurable) – peak area and height
405 nm laser line (Violet)
440 ± 25 nm fluorescence (VL1)
512 ± 13 nm fluorescence (VL2)
603 ± 24 nm fluorescence (VL3)
710 ± 25 nm fluorescence (VL4)
Compatible Stains: Hoescht 34580, others
488 nm laser line (Blue)
Forward Scatter (<15°)
Side (90°) Scatter (SSC)
530 ± 15 nm fluorescence (BL1)
574 ± 13 nm fluorescence (BL2)
695 ± 20 nm fluorescence (BL3)
780 ± 30 nm fluorescence (BL4)
Compatible Stains: picogreen, Sybr-green, FITC, PE/PI, PerCP, many others
Particle Size Range: ~0.2 ~20 um
Sample Delivery
microcentrifuge tubes, flow tubes, microplates, deep well plates, many others; microplates can be automated for high throughput sampling. Sample-throughput rates of of 12.5 µL/min–1 mL/min or 12.5–1,000 µL/min
Data acquisition speeds of up to 35,000 events/sec.