The BD Influx is a high speed sorting flow cytometer and a corner stone instrument of the cytometry facility. Although it can count and characterize cells and populations, its main purpose is for the high speed sorting of single cells through to populations. The instruments specifications are as follows:
Lasers
355nm (100 mW)
457nm (200 mW)
488nm (200 mW)
Optics
488nm laser line
Forward Scatter (perpendicular polarization)
Forward Scatter (parallel polarization)
Side (90 degree) Scatter
520 / 15nm fluorescence
542 / 27nm fluorescence
585 / 40nm fluorescence
692 / 40nm fluorescence
457nm laser line
480 / 40nm fluorescence
650 LP fluorescence
355nm laser line
460 / 50nm fluorescence
670 / 30nm fluorescence
Compatible Stains
Hoechst 33342 and 33358
picogreen
Sybr-green
FITC
PE/PI
PerCP
others
Nozzle sizes
70 µm
100 µm
140 µm
200 µm
Sorting containers
microtubes, slides, 12 x 75-mm tubes, 96-well plates, 24-well plates, 8-well plates, 15 mL, 50mL tubes, and others
Sorting speed
Up to 70,000 events per second, but often much reduced