The BD FACSCalibur is a clinical style flow cytometer typically used for routine counting and characterization of cells and populations. Although it can sort (at very low rates) its main purpose is for population enumeration and characterization. The instruments specifications are as follows:
Laser
488nm (15 mW)
Optics
488nm laser line
Forward Scatter (<15°)
Side (90°) Scatter
530 ± 30 nm fluorescence
585 ± 42 nm fluorescence
>670 nm fluorescence
Compatible Stains: picogreen, Sybr-green, FITC, PE/PI, PerCP, others
Particle Size range: ~0.5 – 20 um
Sorting container: 50mL tubes
Sample delivery
quantitatively using Harvard Apparatus syringe pump
high throughput sampling device (96-well plate format)